Greene County is home to six Civil War sites listed in the Tennessee Civil War Trails program.
The Civil War Trail sites in Greene County include three sites in historic downtown Greeneville, one on the Tusculum College campus and two in the town of Mosheim. The sites include:
- Battle of Blue Springs
- Death of General John Hunt Morgan
- Dickson-Williams Mansion
- Hangings at the Depot
- Pottertown Bridge Burners
- President Andrew Johnson Museum & Library, Tusculum College